This includes the labeling of the dCas9 protein, engineering of sgRNA, and the use of dCas9 orthologs from different bacterial species. CRISPR, clustered regularly interspaced short palindromic ...

CRISPR-dCas9 functions as a master regulator of sgRNA – RNA origami fusion molecules that bring transcription factors to a promoter sequence. Graphics by George Pothoulakis.

Researchers from the Wellcome Sanger Institute and collaborators used human stem cells and neurons to investigate what features influence how well CRISPR activation works for different sets of genes.

The CRISPR gene editing system has offered scientists a powerful tool for studying the effects of altering specific genes. Now, researchers at the Gladstone-UCSF Institute of Genomic Immunology have ...